Journal:

Article Title: Endogenous Lipogenic Regulators of Spore Balance in Aspergillus nidulans †

doi: 10.1128/EC.3.6.1398-1411.2004

Figure Lengend Snippet: A. nidulans strains used in this study

Article Snippet: The ppoC gene was identified by tblastn search of the CEREON Genomics A. nidulans database (Monsanto Microbial Sequence Database), based on the amino acid sequence of linoleate diol synthase (Lds) cloned from Gaeumannomyces graminis ( 28 ) that was used as the query sequence.

Techniques:

Journal:

Article Title: Endogenous Lipogenic Regulators of Spore Balance in Aspergillus nidulans †

doi: 10.1128/EC.3.6.1398-1411.2004

Figure Lengend Snippet: BrlA and NsdD are involved in the regulation of meiospore/mitospore ratio in A. nidulans. (A and B) Gene expression analysis of the spore specific transcriptional regulators brlA (asexual) (A) and nsdD (sexual) (B) in wild-type and ΔppoA, ΔppoC, and ΔppoA ΔppoC mutant strains. (A) Temporal delay of brlA transcripts (α and β) in the conidium-deficient ΔppoC and ΔppoA ΔppoC mutants. (B) nsdD gene is upregulated in the ascospore-overproducing ΔppoC and ΔppoA ΔppoC mutant strains. (C) ppoA and ppoC expression is altered in ΔbrlA, ΔnsdD, and overexpression nsdD (OE::nsdD) strains. Strains were grown in stationary liquid GMM at 37°C, and mycelia were harvested at the indicated time points. Equal loading of total RNA (20 μg) is depicted by ethidium bromide staining of the rRNA.

Article Snippet: The ppoC gene was identified by tblastn search of the CEREON Genomics A. nidulans database (Monsanto Microbial Sequence Database), based on the amino acid sequence of linoleate diol synthase (Lds) cloned from Gaeumannomyces graminis ( 28 ) that was used as the query sequence.

Techniques: Gene Expression, Mutagenesis, Expressing, Over Expression, Staining

Journal:

Article Title: Endogenous Lipogenic Regulators of Spore Balance in Aspergillus nidulans †

doi: 10.1128/EC.3.6.1398-1411.2004

Figure Lengend Snippet: Antagonistic transcriptional regulation of fatty acid biosynthetic genes by PpoA and PpoC. Cultures of A. nidulans wild-type, ΔppoA, ΔppoC, and ΔppoA ΔppoC strains were grown at 37°C in stationary liquid GMM for 72 h and analyzed for the expression profile of fatty acid anabolic genes. The biosynthetic role of each gene is depicted in the flow chart of fatty acid biosynthesis in A. nidulans. Equal loading of total RNA (20 μg) is depicted by ethidium bromide staining of the rRNA.

Article Snippet: The ppoC gene was identified by tblastn search of the CEREON Genomics A. nidulans database (Monsanto Microbial Sequence Database), based on the amino acid sequence of linoleate diol synthase (Lds) cloned from Gaeumannomyces graminis ( 28 ) that was used as the query sequence.

Techniques: Expressing, Staining

Journal:

Article Title: Endogenous Lipogenic Regulators of Spore Balance in Aspergillus nidulans †

doi: 10.1128/EC.3.6.1398-1411.2004

Figure Lengend Snippet: Molecular characterization of the A. nidulans PpoC locus. The ppoC genomic DNA sequence contains 11 introns and 12 exons. The major transcription initiation site was found 136 bp upstream of the first ATG codon, and the polyadenylation site (polyA) is located 128 bp downstream from the ORF stop codon. The deduced translated 1,117-amino-acid polypeptide contains predicted heme peroxidase and cytochrome P-450 domains and a sequence that aligns with the proline knot motif, an essential motif for targeting proteins to lipid bodies. Arrows indicate the conserved proline residues.

Article Snippet: The ppoC gene was identified by tblastn search of the CEREON Genomics A. nidulans database (Monsanto Microbial Sequence Database), based on the amino acid sequence of linoleate diol synthase (Lds) cloned from Gaeumannomyces graminis ( 28 ) that was used as the query sequence.

Techniques: Sequencing

Journal:

Article Title: Endogenous Lipogenic Regulators of Spore Balance in Aspergillus nidulans †

doi: 10.1128/EC.3.6.1398-1411.2004

Figure Lengend Snippet: Psi factor composition of mycelia of A. nidulans oxylipin mutants

Article Snippet: The ppoC gene was identified by tblastn search of the CEREON Genomics A. nidulans database (Monsanto Microbial Sequence Database), based on the amino acid sequence of linoleate diol synthase (Lds) cloned from Gaeumannomyces graminis ( 28 ) that was used as the query sequence.

Techniques: Mutagenesis

Journal:

Article Title: Endogenous Lipogenic Regulators of Spore Balance in Aspergillus nidulans †

doi: 10.1128/EC.3.6.1398-1411.2004

Figure Lengend Snippet: Fatty acid composition of mycelia of A. nidulans oxylipin mutants a

Article Snippet: The ppoC gene was identified by tblastn search of the CEREON Genomics A. nidulans database (Monsanto Microbial Sequence Database), based on the amino acid sequence of linoleate diol synthase (Lds) cloned from Gaeumannomyces graminis ( 28 ) that was used as the query sequence.

Techniques: Mutagenesis

Journal:

Article Title: Endogenous Lipogenic Regulators of Spore Balance in Aspergillus nidulans †

doi: 10.1128/EC.3.6.1398-1411.2004

Figure Lengend Snippet: ppoA and ppoC genes are essential for balancing conidiophore/cleistothecia formation. Cultures of A. nidulans wild-type (B1, C1, and D1), ΔppoA (B2, C2, and D2), ΔppoC (B3, C3, and D3) and ΔppoA ΔppoC (B4, C4, and D4) were grown at 37°C on solid GMM. (A) Deletion of ppoC delays conidiophore formation. Five-day-old cultures of point-inoculated strains (inoculum, 103 conidia) under light conditions. (B to D) Induction of the sexual sporulation and suppression of the asexual fruiting bodies in ΔppoC and ΔppoA ΔppoC strains. The opposite is observed in the ΔppoA mutant. Each strain was inoculated with 106 conidia/plate, and cultures were grown for 2 days under dark (B), 8 days under dark (C), and 8 days under light (D) conditions. Black balls are cleistothecia (“Cl” in panel D3), fuzzy balls are cleistothecia initials (“Cl In” in B4), and smaller green spheres are conidiophore heads.

Article Snippet: The ppoC gene was identified by tblastn search of the CEREON Genomics A. nidulans database (Monsanto Microbial Sequence Database), based on the amino acid sequence of linoleate diol synthase (Lds) cloned from Gaeumannomyces graminis ( 28 ) that was used as the query sequence.

Techniques: Mutagenesis

Journal:

Article Title: Endogenous Lipogenic Regulators of Spore Balance in Aspergillus nidulans †

doi: 10.1128/EC.3.6.1398-1411.2004

Figure Lengend Snippet: Deletion of ppoC induces sexual development in liquid cultures. Cultures of A. nidulans wild-type (A), ΔppoC (B), and ΔppoA ΔppoC (C) were grown at 37°C in liquid GMM. Abundant presence of Hülle cells after 2 days growth in liquid cultures of ΔppoC and ΔppoA ΔppoC strains (B and C). The double mutant was able to produce mature and fertile cleistothecia after 2 days of growth (C2 and C3). Hu, Hülle cells; Cl, cleistothecium; As, ascospores.

Article Snippet: The ppoC gene was identified by tblastn search of the CEREON Genomics A. nidulans database (Monsanto Microbial Sequence Database), based on the amino acid sequence of linoleate diol synthase (Lds) cloned from Gaeumannomyces graminis ( 28 ) that was used as the query sequence.

Techniques: Mutagenesis

Journal:

Article Title: Endogenous Lipogenic Regulators of Spore Balance in Aspergillus nidulans †

doi: 10.1128/EC.3.6.1398-1411.2004

Figure Lengend Snippet: Proposed model depicting the genetic relationship between PpoC, PpoA, BrlA, and NsdD to establish the meiotic/mitotic ratio in A. nidulans. PpoA and PpoC are involved in linoleic and oleic acid oxylipin production, respectively. BrlA and NsdD are transcription factors regulating mitotic and meiotic development, respectively. The numbers of individual conidiophores and cleistothecia are indicative of the asexual/sexual ratio in each mutant.

Article Snippet: The ppoC gene was identified by tblastn search of the CEREON Genomics A. nidulans database (Monsanto Microbial Sequence Database), based on the amino acid sequence of linoleate diol synthase (Lds) cloned from Gaeumannomyces graminis ( 28 ) that was used as the query sequence.

Techniques: Mutagenesis

Journal: Frontiers in Microbiology

Article Title: Exploring the potential of ume-derived proanthocyanidins: novel applications for blueberry preservation

doi: 10.3389/fmicb.2023.1265993

Figure Lengend Snippet: Bacteriostatic effect of UPPP on S. aureus , E. coli and A. niger . [ (A–C) Blank controls for S. aureus , E. coli and A. niger , respectively; (D) S. aureus ; (E) E. coli ; (F) A. niger ].

Article Snippet: Ume (purchased from Dachuan, Sichuan, China), sterile TTC solution (0.5%; Guangdong Huankai Biotechnology Co., Ltd.), Staphylococcus aureus ( S. aureus ; ATCC 29231, GenBank: U77328 S. aureus staphylokinase gene, partial cds.), Escherichia coli ( E. coli ; ATCC 25922, GenBank: AF038431 E. coli DNA gyrase A (gyrA) gene, partial cds.), Aspergillus niger ( A. niger ; ATCC 16888, GenBank: FJ195350 ITS including 5.8S rRNA gene; purchased from Guangdong Microbial Strain Conservation Center, China), POD kit, SOD kit (Beijing Solepol Technology Co., Ltd., China), Handy Plate® Aerobic Count Plate (HP001, Guangdong Huankai Biotechnology Co., Ltd.).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Exploring the potential of ume-derived proanthocyanidins: novel applications for blueberry preservation

doi: 10.3389/fmicb.2023.1265993

Figure Lengend Snippet: Inhibitory effect of UPPP on three experimental bacteria.

Article Snippet: Ume (purchased from Dachuan, Sichuan, China), sterile TTC solution (0.5%; Guangdong Huankai Biotechnology Co., Ltd.), Staphylococcus aureus ( S. aureus ; ATCC 29231, GenBank: U77328 S. aureus staphylokinase gene, partial cds.), Escherichia coli ( E. coli ; ATCC 25922, GenBank: AF038431 E. coli DNA gyrase A (gyrA) gene, partial cds.), Aspergillus niger ( A. niger ; ATCC 16888, GenBank: FJ195350 ITS including 5.8S rRNA gene; purchased from Guangdong Microbial Strain Conservation Center, China), POD kit, SOD kit (Beijing Solepol Technology Co., Ltd., China), Handy Plate® Aerobic Count Plate (HP001, Guangdong Huankai Biotechnology Co., Ltd.).

Techniques: Bacteria, Inhibition, Control

Journal: Frontiers in Microbiology

Article Title: Exploring the potential of ume-derived proanthocyanidins: novel applications for blueberry preservation

doi: 10.3389/fmicb.2023.1265993

Figure Lengend Snippet: Inhibitory effect of UPPP on three experimental bacteria.

Article Snippet: Ume (purchased from Dachuan, Sichuan, China), sterile TTC solution (0.5%; Guangdong Huankai Biotechnology Co., Ltd.), Staphylococcus aureus ( S. aureus ; ATCC 29231, GenBank: U77328 S. aureus staphylokinase gene, partial cds.), Escherichia coli ( E. coli ; ATCC 25922, GenBank: AF038431 E. coli DNA gyrase A (gyrA) gene, partial cds.), Aspergillus niger ( A. niger ; ATCC 16888, GenBank: FJ195350 ITS including 5.8S rRNA gene; purchased from Guangdong Microbial Strain Conservation Center, China), POD kit, SOD kit (Beijing Solepol Technology Co., Ltd., China), Handy Plate® Aerobic Count Plate (HP001, Guangdong Huankai Biotechnology Co., Ltd.).

Techniques: Bacteria, Concentration Assay